Part:BBa_K1486043

Leucine Zipper + split rLuc

Purpose of the Biobrick

This construct aimed to test the efficiency of split Renilla luciferase, by performing a complementation assay using leucine zippers.

Experiment: Complementation assay with leucine zippers

Leucine zipper is a structural motif in many proteins. It is often found in transcription factors and function as a dimerization domain.

To proceed to this complementation assay, we built a construct to test split Renilla Luciferase. Both parts (N-terminal and C-terminal) were each fused to a different zipper. We used the full luciferase (BBa_K1486022) as a positive control..

As shown in the graph below, we couldn't really observe a high signal for our complementation assay. However, the signal being higher than the blanks, it is an encouraging sign that the split luciferase can be used for other experiments of this kind.

Associated Biobricks

• LZip ( https://parts.igem.org/Part:BBa_K1486042 )

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961